Levels of cyclooxygenase-2 and proinflammatory cytokines in lipopolysaccharide- and honokiol-treated BALB/c mice. BALB/c mice administered with different doses of HNK and 5 mg/kg body weight of LPS were analyzed by Cox-2 immunoblotting and CBA analysis. (A) Decrease in the levels of Cox-2 in mice treated with different doses of honokiol. Graph represents fold change in Cox-2 levels relative to untreated control condition. (B-D) CBA analysis of brain homogenates from BALB/c mice in different conditions. There was a significant decrease in the levels of MCP-1 (B), IL-6 (C) and TNF-α (D) in mice treated with different doses of HNK. Absolute values of cytokines are represented in pg/mL. Data represent mean ± SEM of five animals in each group. *P < 0.05 and **P < 0.01 in comparison to LPS-treated values.
Kaushik et al. Journal of Neuroinflammation 2012 9:57 doi:10.1186/1742-2094-9-57