JEV-induced c-Src/PDGFR/PI3K/Akt is mediated via ROS in RBA-1 cells. (A) Cells were pretreated with APO (100 μM), DPI (10 μM), or NAC (10 mM) for 1 h, and then stimulated with JEV for 5 min. The cell lysates were subjected to immunoprecipitation using an anti-c-Src antibody. The immunoprecipitates were analyzed by western blot analysis using an anti-c-Src, anti-phospho-c-Src, or anti-phospho-PDGFR antibody. (B, C) Cells were pretreated with APO (100 μM), DPI (10 μM), or NAC (10 mM) for 1 h, and then incubated with JEV for 30 min. The cell lysates were analyzed by western blot using an anti-phospho-PDGFR, anti-phospho-Akt, or anti-GAPDH antibody. Data are expressed as mean ± S.E.M. for five independent experiments. *P < 0.05; #P < 0.01, as compared with the cells exposed to JEV alone.
Yang et al. Journal of Neuroinflammation 2012 9:12 doi:10.1186/1742-2094-9-12