Figure 7.

DJ-1−/− mice display blunted oxidative stress responses to prolonged, serial administration of low-dose systemic lipopolysaccharide relative to wild-type mice. Real-time quantitative PCR analyses of microdissected midbrain tissue from mice treated with low-dose lipopolysaccharide (LPS) (or saline) for 6 months (mo) measured expression levels of NF-E2 related factor (Nrf2), heme-oxygenase-1 (HO-1), inducible nitric oxide synthase (iNOS), superoxide dismutase-1 (SOD-1), and superoxide dismutase-2 (SOD-2). *,**Significant difference from wild-type saline; #Significant difference from DJ-1−/− saline; +Significant difference between the LPS conditions. Bars represent mean ± SEM; n = 3 to 4 per group. A two-way analysis of variance was performed with Tukey’s HSD post hoc at *P < 0.05 (Nrf2 treatment effect: F(1,10) = 7.96, P = 0.0181, genotype effect: F(1,10) = 8.28, P = 0.016; HO-1 treatment effect: F(1,10) = 7.56, P = 0.0205, genotype effect: P = 0.166; iNOS not significant: treatment effect, P = 0.2474, genotype effect, P = 0.183; however, a significant relationship between treatments and genotype did exist (F(1,10) = 5.318, P = 0.044); SOD-1 treatment effect: F(1,10) = 14.67, P = 0.0033, genotype effect: F(1,10) = 9.47, P = 0.012 SOD-2 treatment effect: not significant, P = 0.5175, genotype effect: F(1,10) = 7.08, P = 0.024).

Nguyen et al. Journal of Neuroinflammation 2013 10:50   doi:10.1186/1742-2094-10-50
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