Figure 4.

DJ-1−/− mice do not display increased vulnerability to nigral dopamine neuron loss induced by repeated low-dose systemic lipopolysaccharide compared to wild-type mice. Unbiased stereological analysis indicates that DJ-1−/− mice exposed to 3 or 6 months of low-dose systemic lipopolysaccharide (LPS) or to 3 months of low dose systemic LPS followed by a 3 month wait before analysis do not display a significant reduction of tyrosine hydroxylase (TH) or NeuN immunopositive neurons in (A) the substantia nigra pars compacta (SNpc) or (B) the ventral tegmental area (VTA). Error bars represent SEM; analysis includes n = 3 to 7 per group in final cohorts due to attrition. Asterisks indicate significant differences compared with wild-type, saline-treated mice by three-way analysis of variance followed by Tukey’s HSD post hoc test at P < 0.05. For SNpc TH neurons: genotype F(1, 47) = 7.89, P = 0.007; treatment F(1, 47) = 0.01, P = 0.904. For SNpc NeuN neurons: genotype F(1, 47) = 1.05, P = 0.311; treatment F(1, 47) = 0.0.03, P = 0.858. For VTA TH neurons: genotype F(1, 47) = 8.57, P = 0.005, post hoc analysis reveal no significant differences; treatment F(1, 47) = 0.39, P = 0.536. For VTA NeuN neurons: genotype F(1, 47) = 1.26, P = 0.268; treatment F(1, 47) = 01.28, P = 0.269.

Nguyen et al. Journal of Neuroinflammation 2013 10:50   doi:10.1186/1742-2094-10-50
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