Table 2

Summary of antibodies used for immunohistochemistry
Antibody Manufacturer Dilution In combination with
Rat anti-mouse C1q Hycult Biotechnology1 1:1,000 GFAP, Iba-1 and ChAT
Rat anti-mouse C3b Hycult Biotechnology1 1:50 GFAP, Iba-1 and ChAT
Mouse anti-mouse C5 Hycult Biotechnology1 1:1,000 GFAP, CD11b, ChAT
Rat anti-mouse CD88 AbD Serotec2 1:250 GFAP, Iba-1, ChAT
Mouse anti-mouse GFAP BD Biosciences3 1:1,000 C1q, C3b, C5, CD88
Rabbit anti-mouse Iba-1 Wako4 1:400 C1q, C3b and CD88
Rat anti-mouse CD11b Abcam5 1:300 C5
Goat anti-mouse ChAT Chemicon6 1:100 C1q, C3b, C5, CD88

GFAP, glial fibrillary acidic protein; ChAT, choline acetyltransferase; Iba-1, ionized calcium-binding adapter molecule-1;1Hycult Biotechnology, Netherlands; 2 AbD Serotec; 3 BD Biosciences, Sydney, NSW, Australia; 4 Wako, Osaka, Japan; 5 Abcam, Sydney, NSW, Australia; 6 Chemicon, Billerica, MA, USA.

Lee et al.

Lee et al. Journal of Neuroinflammation 2013 10:119   doi:10.1186/1742-2094-10-119

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