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Prolonged oral cannabinoid administration prevents neuroinflammation, lowers β-amyloid levels and improves cognitive performance in Tg APP 2576 mice

Ana María Martín-Moreno1,2,7, Begoña Brera1,2, Carlos Spuch2,3,8, Eva Carro2,3, Luis García-García4, Mercedes Delgado4, Miguel A Pozo4,5, Nadia G Innamorato2,6, Antonio Cuadrado2,6 and María L de Ceballos1,2*

1 Neurodenegeration Group, Dept. of Cellular, Molecular and Developmental Neurobiology, Instituto Cajal, CSIC, Doctor Arce, 37, Madrid, 28002, Spain

2 Centre for Biomedical Research on Neurodegenerative Diseases (CIBERNED), Madrid, Spain

3 Neuroscience Group, Research Institute Hospital 12 de Octubre, Av. de Córdoba s/n Madrid, 28041, Spain

4 CAI de Cartografía Cerebral, Instituto Pluridisciplinar, UCM, Paseo Juan XXIII 1, Madrid, 28040, Spain

5 PET Technology Institute, Manuel Bartolomé Cossio 10, Madrid, 28040, Spain

6 Departamento de Bioquímica, Instituto de Investigaciones Biomédicas "Alberto Sols"UAM-CSIC, Facultad de Medicina, Universidad Autónoma de Madrid, Arturo Duperier 4, Madrid, 28029, Spain

7 Current Address: MD Anderson Cancer Center, Arturo Soria 270, 28033 Madrid, Spain

8 Current Address: Department of Pathology and Neuropathology, University Hospital of Vigo (CHUVI), Vigo, Spain

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Journal of Neuroinflammation 2012, 9:8 doi:10.1186/1742-2094-9-8

Published: 16 January 2012

Additional files

Additional file 1:

Co-localization of CB2 and Iba-1 inmmunostaining in quinolinic acid injected striatum. Iba-1 positive microglia in striatum also express CB2 receptor protein ipsilateral to the toxin injection, but was absent in the contralateral (unlesioned) striatum. Tg APP microglial cells are devoid of CB2 immunostaining.

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Additional file 2:

Additional methods. (quinolinic acid striatal lesion and blockade of CB2 antibodies with CB2 blocking peptide) and additional file 1, which includes the antibodies, commercial source and dilution, used in immunohistochemistry and Western blotting.

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Additional file 3:

Blockade of CB2 antibodies 744 and 746 with CB2 antigenic peptide. The CB2 peptide completely blocked immunostaining.

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