Effect of honokiol on lipopolysaccharide-stimulated BV-2 cells. Total cellular extract isolated from cells treated with 500 ng/mL of LPS and different doses of HNK for 12 hours were analyzed by immunoblotting. (A) Significant decrease in iNOS levels upon treatment with 1 μM and 10 μM HNK in LPS-treated cells with respect to LPS alone-treated cells. (B) Significant decrease in the expression of Cox-2 upon treatment with 10 μM HNK. The graphs represent fold change in protein levels relative to untreated controls. (C-E) CBA analysis of protein extract from BV-2 cells treated with LPS as well as in LPS + HNK conditions. CBA revealed a significant decrease in BV-2 cells treated with different doses of HNK in the levels of MCP-1 (C), IL-6 (D) and TNF-α (E) with respect to LPS. Absolute values of cytokines are represented in pg/mL. Data represent mean ± SEM from three independent experiments performed in duplicate. *P < 0.05 and **P < 0.01 in comparison to LPS-treated values.
Kaushik et al. Journal of Neuroinflammation 2012 9:57 doi:10.1186/1742-2094-9-57