Table 1

Confirmation of the ERα agonist-regulated immunity/inflammation genes by quantitative real-time PCR.

GENE

MICROARRAY

REAL-TIME PCR

Symbol

Name

probe set

FC

TaqMan ID

RQ


S100a9

S100 calcium binding protein A9

1387125_at

3.655

Rn00585879_m1

2.029

S100a8

S100 calcium binding protein A8

1368494_at

3.458

Rn00587579_g1

2.558

RT1-Aw2

RT1 class Ib

1388202_at

0.291

Rn03034964_u1

0.658

RatNP-3b

defensin ratNP-3 precursor

13700791_at

3.317

Rn01478511_gH

10.568

IgG-2a

immonoglobulin G

1370394_at

3.204

Rn01429839_g1

9.257

Np4

defensin NP-4 precursor

1370470_at

3.031

Rn00597762_g1

2.268

Defa

defensin, alpha 5, Paneth cell-specific

1387943_at

2.732

Rn02607254_g1

32.763

Prg2

proteoglycan 2

1387633_at

2.412

Rn00581137_m1

1.257

Camp

cathelicidin antimicrobial peptide

1393603_at

2.297

Rn01446021_g1

1.521

Mcpt9

mast cell protease 9

1368501_s_at

2.235

Rn00755366_g1

5.825

Fcgr2b

Fc fragment of IgG, receptor

1371079_at

0.451

Rn00598391_m1

0.649

Igh-1a

immunoglobulin heavy chain

1388272_at

2.144

Cd74

CD74

1367679_at

0.473

Rn00565062_m1

0.685

Fcnb

ficolin beta

1387378_at

2.071

Rn00586231_m1

1.922

Mcpt8

mast cell protease 8

1369586_at

2.056

Rn01789238_g1

3.784

Igj

immunoglobulin joining chain

1383163_at

1.945

Rn01768305_m1

1.204

Igha

immunoglobulin heavy chain, alpha

1371262_at

1.866

RT1-Aw2

RT1 class Ib

1388203_x_at

0.536

Rn03034964_u1

0.658

Serping1

C1-Inhibitor

1372254_at

0.559

Rn01485600_m1

0.690

C3

complement C3

1368000_at

0.559

Rn00566466_m1

0.743

RT1-N1

RT1 class Ib, locus N1

1387839_at

0.570

Rn00561858_m1

0.654

Lrrc8a

leucine rich repeat containing 8 family

1382920_at

1.670


Transcriptional regulation of twenty-one immunity genes determined by the top100 probe sets of microarray analysis was confirmed by real-time PCR. FC, fold change; RQ, relative quantity.

Sárvári et al. Journal of Neuroinflammation 2011 8:82   doi:10.1186/1742-2094-8-82

Open Data