Spheroid myelinating cultures were prepared from fetal rat telencephalon and demyelinated with lysophosphotidyl choline on DIV 25-29 in the presence or absence of AUY954, an S1P1 agonist, or BAF312, an S1P1 and S1P5 agonist. Myelin basic protein (A) and neurofilament (B) levels were measured using protein specific ELISA following demyelination. Results were analysed by ANOVA with Holm-Sidak post test. * = p < 0.05; ** = p < 0.01.
Jackson et al. Journal of Neuroinflammation 2011 8:76 doi:10.1186/1742-2094-8-76