Additional file 1.
Genotyping of the presence of TLR3. The presence/absence of TLR3 in TLR3KO-SJL and the littermate mice (NLM) were typed based on the electrophoresis patterns of TLR3 and neomycin resistant genes. PCR products from tail genomic DNA of NLM and TLR£KO-SJL mice were determined using PCR-based genotyping analysis, established by the Jackson Laboratory. TLR3 primers (5'-ACT CCT TTG GGG GAC TTT TG-3 and 5'-CAG GTT CGT GCA GAA GAC AA-3') and Neo generic primers (5'-CTT GGG TGG AGA GGC TAT TC-3' and 5'-AGG TGA GAT GAC AGG AGA TC-3') were used for PCR.
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Jin et al. Journal of Neuroinflammation 2011 8:178 doi:10.1186/1742-2094-8-178