IL-1β induces expression of IL-1α, ApoE, and βAPP. Immunofluorescence was performed on tissue sections from the neocortex or hippocampus (CA1) of unoperated rats (n = 4) (Control), from rats implanted with a pellet containing vehicle alone (n = 6) (Sham), and from rats implanted with a slow-release pellet containing 100 ng IL-1β (n = 6) (IL-1β Pellet). Primary antibodies were A: ApoE, B: IL-1α, or C: βAPP; the red immunofluorescence signal is countered by a blue DAPI stain of cell nuclei. Immunofluorescence was quantified as described in Materials and Methods, and is expressed as mean ± SEM. All treatment conditions were significantly different from each other except with regard to IL-1α, where only the comparison of IL-1β pellet to the other two treatments was significant (*p ≤0.05).
Liu et al. Journal of Neuroinflammation 2011 8:175 doi:10.1186/1742-2094-8-175