Table 1 |
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|
Primary antibody and antibody/fluorochrome-conjugates against various immune mediators, B. burgdorferi, and cell phenotypes |
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| MEDIATOR/CELL TYPE |
PRIMARY AB/SOURCE |
ISOTYPE |
DILUTION/CONCENTR. |
|
|
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| IL-6 |
anti-human IL-6 (Abcam Inc. Cambridge, MA) |
Rabbit IgG |
1:1000 |
|
|
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| IL-8 |
anti-human IL-8 RDI Flanders, NJ |
Rabbit IgG |
10 μg/ml |
|
|
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| CCL2 |
anti-human CCL2 (BD) |
Mouse IgG1 |
10 μg/ml |
|
|
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| anti-human CCL2 (Abcam) |
Rabbit IgG |
1:10 |
|
|
|
|||
| CXCL13 |
anti-human CXCL13 (R&D) |
goat IgG |
5 μg/ml |
|
|
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| B. burgdorferi |
anti-whole cell preparation (Accurate Chemicals, Westbury, NY) |
Rabbit IgG |
1:250 |
|
|
|||
| anti- B. burgdorferi-FITC (Kirkegaard and Perry, Gaithersburg, MD) |
Goat |
1:10 |
|
|
|
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| Astrocyte |
anti-human GFAP-cy3 (Sigma St Louis, MO) |
Mouse IgG1 |
1:200 |
|
|
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| anti-human GFAP (Sigma) |
Mouse IgG1 |
1:200 |
|
|
|
|||
| Astrocyte, Oligodendrocyte, Schwann cell, satellite cell |
anti-bovine S-100 (Sigma) |
Rabbit IgG |
1:1000 |
|
|
|||
| Microglia |
anti-Iba1 synthetic peptide (Wako Pure Chemicals, Richmond, VA) |
Rabbit IgG |
1:100 |
|
|
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| Endothelial cells |
anti-human GLUT-1 (Chemicon) |
Rabbit IgG |
1:50 |
|
|
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| Neuron |
anti-mouse NeuN (Chemicon) |
Mouse IgG1 |
1:20 |
|
|
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| T cell |
anti-human CD3 (Dako, Carpinteria, CA) |
Mouse IgG2a |
1:10 |
|
|
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| B cell |
anti-human CD20 (Dako) |
Mouse IgG1 |
1:200 |
|
|
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| Macrophage |
anti-human CD68 (Dako) |
Mouse IgG1 |
1:50 |
Ramesh et al. Journal of Neuroinflammation 2009 6:23 doi:10.1186/1742-2094-6-23 |
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