Figure 4.

Cerebral cortical cultures were pre-incubated for 32 h with acetaminophen (0–300 μM) (APAP) and oxidatively stressed with menadione (5 μM for 4 h). Cytokines (a) IL-1α (b) IL-1β and (c) TNFα released into the supernatants were determined by indirect ELISA. Data are mean ± SD from 3 separate experiments. **p < 0.01 vs. menadione alone; ***p < 0.001 vs. menadione alone; #p < 0.001 vs. control.