Figure 4.

Lower IL-1β production with a TLR4 agonist in the ASD test group following LPS pre-treatment. In experiments shown in Figures 4, 5 and 6, PBMCs were treated with LPS (0.1 μg/ml) overnight and re-stimulated with TLR agonists as described in Figure 1 after replacing the culture medium. IL-1β production was lower in the ASD test group in this setting than in the normal case controls.