Figure 2.

Effect of Th1 cytokines on oligodendrocyte precursors. CNS mixed CNS glial cultures were incubated with Th1 cytokines or additional medium and fixed as in Figure 1. Cultures were then incubated with mouse A2B5 (IgM) and mouse anti rat MHC class II (IgG) followed by Alexa 488-conjugated goat anti-mouse IgM and Cy 3-conjugated donkey anti-mouse IgG. Cultures were examined by indirect immunofluoresence as above. Compared to control, the Th1 treated A2B5+ oligodendrocyte precursors have a much more mature appearance including more extensive process formation although the cells still express A2B5, but do not express MHC class II.