|
Pre-treatment with IFN-γ increases prostaglandin E2 production in response to amyloid-β or HuPrP82-146. SH-SY5Y neuroblastoma cells were pre-treated for 24 hours with 1 ng/ml cytokines as shown prior to the addition of amyloid-β or PrP peptides. Cells were lysed 24 hours later and total prostaglandin E2 levels were measured. Each value given represents the mean ± SD from triplicate experiments repeated twice (6 observations). ** = Prostglandin E2 levels significantly higher than untreated neurons incubated with amyloid-β1-42 (p < 0.05). |
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| Prostaglandin E2 (pg/ml) |
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|
|
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| Amyloid-β1-42 |
Amyloid-β42-1 |
HuPrP82-146 |
HuPrP82-146sc |
|
|
|
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| Control medium |
241 ± 62 |
< 50 |
187 ± 40 |
< 50 |
| IFN-γ |
443 ± 112** |
66 ± 38 |
303 ± 55** |
< 50 |
| IL-1β |
228 ± 54 |
< 50 |
202 ± 46 |
< 50 |
| IL-6 |
218 ± 66 |
< 50 |
210 ± 48 |
55 ± 35 |
| TNFα |
255 ± 82 |
< 50 |
214 ± 33 |
< 50 |
Bate et al. Journal of Neuroinflammation 2006 3:7 doi:10.1186/1742-2094-3-7 |
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