|
IFN-γ treated neurons show increased sensitivity to HuPrP82-146. Neurons treated with 1 ng/ml IFN-γ for 24 hours prior to the addition of neurotoxins as shown. Cell survival was determined 24 hours later using the WST-1 test. Each value is the mean percentage cell survival ± SD from triplicate experiments repeated 3 times (9 observations). ** = Neuronal survival significantly less than untreated neurons incubated with HuPrP82-146 (p < 0.05). |
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| Neuronal survival (%) |
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|
|
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| conc |
Control |
IFN-γ |
|
|
|
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| HuPrP82-146 (μM) |
40 |
48 ± 6 |
12 ± 7** |
| 10 |
64 ± 5 |
22 ± 8** |
|
| 2.5 |
92 ± 9 |
41 ± 9** |
|
| Staurosporine (ng/ml) |
20 |
37 ± 4 |
40 ± 6 |
| 5 |
78 ± 6 |
72 ± 8 |
|
| 1.25 |
92 ± 3 |
95 ± 4 |
|
| Hydrogen peroxide (μM) |
1 |
22 ± 5 |
18 ± 7 |
| 0.25 |
58 ± 7 |
55 ± 6 |
|
| 0.06 |
87 ± 6 |
89 ± 9 |
|
Bate et al. Journal of Neuroinflammation 2006 3:7 doi:10.1186/1742-2094-3-7 |
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