Table 1

Treatment with IFN-γ reduces neuronal survival following incubation with amyloid-β1-42. SH-SY5Y cells or primary neuronal cell cultures were pre-treated with IFN-γ (1 ng/ml) for 24 hours prior to the addition of amyloid-β peptides as shown. Cell survival was determined using the WST-1 test after 24 hours (cell lines) or 4 days (primary neuronal cultures). Each value is the mean percentage cell survival ± SD from triplicate experiments repeated 3 times (9 observations). ** = Neuronal survival significantly less than untreated neurons incubated with amyloid-β1-42 (p < 0.05).

% Neuronal Survival
Cell Type
 Amyloid-β1-42
 Amyloid-β42-1

Control
 IFN-γ
 Control
 IFN-γ
SH-SY5Y cells
 62 ± 4
 33 ± 9**
 101 ± 4
 98 ± 7
Cortical neurons
 68 ± 7
 36 ± 7**
 98 ± 3
 96 ± 5
Cerebellar neurons
 79 ± 4
 58 ± 11**
 101 ± 8
 102 ± 8

Bate et al. Journal of Neuroinflammation 2006 3:7   doi:10.1186/1742-2094-3-7