Figure 6.

Linear formation of reaction product over time in plasma samples from rats injected with CHEC-9 or vehicle. The rats were restrained for blood sampling and then injected with 100 μg peptide or equivalent volume of vehicle under the skin of the back. The reactions of plasma showed a roughly linear increase in product for the period of measurement using 15 μM of the 10-pyrene substrate. Therefore the concentration of active enzyme in the plasma is proportional to the rate of the reaction. Examples shown are samples from peptide and vehicle-treated rats taken 30 minutes after treatment, and a plasma sample from a vehicle injected animal prior to treatment. RFU = relative fluorescent units.