ApoE secretion following LPS stimulation. Mouse cerebral primary astrocyte cultures were incubated in serum-free medium with 100 ng/ml LPS or vehicle (PBS) for 24 hours. 20 μl of conditioned medium was collected at 3, 6, 12, and 24 hrs after exposure, spun briefly, mixed with 4 μl of 6X sample buffer (0.35 M Tris, 30% glycerol, 10% SDS, 0.93 g DTT, and 1.2 mg bromophenol blue), and relative concentrations of apoE determined by Western blotting. Human high-density lipoprotein (hHDL) prepared in the same sample buffer was included as a positive control.
Maezawa et al. Journal of Neuroinflammation 2006 3:10 doi:10.1186/1742-2094-3-10