Figure 2.

Typical responses of cultured AD microglia to pre-aggregated Aβ1-42 spots dried down to the well floor. A) Micrograph of Aβ spot (light brown stain) and LN3 immunoreactive microglia (blue stain) 3 days postplating (vehicle control) (4 × objective). B) Graphic summary of microglial distributions at 3 days postplating (pooled data over all conditions). C) Parallel well 9 days postplating (vehicle control) (4 × objective). Wells seeded with microglia but without Aβ spots exhibited only random distributions of cells (not shown). D) Graphic summary of microglial distributions at Day 9 (pooled data over all conditions). Similar and highly significant shifts over time were observed in all treatment conditions when Aβ spots were present (see text).