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Open Access Highly Accessed Research

Rosmarinic acid protects against experimental diabetes with cerebral ischemia: relation to inflammation response

Haiyun Luan, Zechun Kan, Yong Xu, Changjun Lv* and Wanglin Jiang*

Author Affiliations

School of Pharmaceutical Sciences and Institute of Material Medica, Binzhou Medical University, Yantai, 264003, People's Republic of China

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Journal of Neuroinflammation 2013, 10:28  doi:10.1186/1742-2094-10-28

Published: 17 February 2013

Abstract

Background

Inflammatory activation plays a vital role in the pathophysiological mechanisms of stroke, exerting deleterious effects on the progression of tissue damage and may lead to the vascular damage in diabetes. The objectives of this study were to determine the effects of rosmarinic acid (RA) on a cultured neuronal cell line, SH-SY5Y in vitro and experimental ischemic diabetic stroke in vivo.

Methods

For oxygen-glucose deprivation (OGD) and tumor necrosis factor-α (TNF-α) stimulated SH-SY5Y cell line in vitro, SH-SY5Y cells were incubated with RA. For an in vivo experiment, diabetic rats were subjected to middle cerebral artery occlusion (MACO) for 40 minutes followed by reperfusion for 23 h.

Results

Treatment of SH-SY5Y cells with RA reduced the OGD-induced apoptosis and cytotoxicity, blocked TNF-α-induced nuclear transcription factor κB (NF-κB) activation, and decreased high-mobility group box1 (HMGB1) expression. At doses higher than 50 mg/kg, RA produced a significant neuroprotective potential in rats with ischemia and reperfusion (I/R). RA (50 mg/kg) demonstrated significant neuroprotective activity even after delayed administration at 1 h, 3 h and 5 h after I/R. RA 50 mg/kg attenuated histopathological damage, decreased brain edema, inhibited NF-κB activation and reduced HMGB1 expression.

Conclusion

These data show that RA protects the brain against I/R injury with a favorable therapeutic time-window by alleviating diabetic cerebral I/R injury and attenuating blood–brain barrier (BBB) breakdown, and its protective effects may involve HMGB1 and the NF-κB signaling pathway.

Keywords:
RA; Cerebral ischemia; Diabetes; Nuclear factor kappaB; Inflammatory response; High-mobility group box1